Phytochemical screening, antioxidant and antimicrobial activities of hexane, ethylacetate and methanol crude extracts of acalypha ciliata plant was studied in this work. The results showed the efficacy of ethonolic extracts showing the presence of more phytochemicals in comparison to aqueous extracts. Pdf phytochemical screening of the medicinal plants of nepal. Phytochemical screening weighed quantity of the various extracts, were subjected to preliminary phytochemical screening using standard methods27.
Phytochemical screening, antioxidant and antimicrobial. The preliminary phytochemical screening of methanolic extract of cardiospermum halicacabum showed the presence of bioactive components like alkaloids, flavonoids, saponins, tannins and glycosides was absent in the plant extract. The preliminary phyochemical screening of methanol and ethanol extracts of. Phytochemical screening of selected indigenous medicinal. Development of a brownish green or a blueblack colouration indicated the presence of tannins. The extracts of the dry powdered leaves of alstoniamacrophyllawere analyzed. The leaves of the plant were subjected to solvent extraction for ethanolic and aqueous extracts. In the present study etoac, meoh, and aqueous extracts of eremurus himalaicus were evaluated for hypoglycaemic effect in normal rats using both oral glucose tolerance test and 14day oral administration study.
Phytochemical and physicochemical screening was also done. The clustering analyses of the phytochemical screening are presented in fig. Phytochemical screening, total phenolics and antioxidant. Antimicrobial screening of plant extracts and phytochemicals, then, represents a starting point for antimicrobial drug discovery cseke et al. Wadood a, ghufran m, jamal sb, naeem m, khan a, et al.
Preliminary phytochemical screening the phytochemical screening of the extracts was conducted using standard procedures described by trease and evans 21. Phytochemical and biological investigations on medicinal plants. Phytochemical screening of various extracts of psidium guajava linn. All extracts in this study could inhibit the growth of s. Preliminary phytochemical screening, isolation and. Phytochemical screening, cytotoxic and antimicrobial. Preliminary phytochemical screening, quantitative analysis of. Phytochemical screening of medicinal plants research. Phytochemical analysis and assessment of antioxidant and. This extraction was done by taking 20 gm of dried plant powder. Preparation of plant extracts for phytochemical analysis the phytochemical extraction was performed using organic solvent extraction as well as aqueous extraction. Antibacterial properties and phytochemical screening of.
Conclusion phytochemical screening of petroleum ether, chloroform, ethanol, aqueous and hydroalcoholic extracts revealed the presence flavonoids, tannins. Phytochemical screening of the extracts was then conducted using qualitative chemical analysis. Preliminary phytochemical screening, quantitative analysis. Plantderived substances have recently become of great interest owing to their versatile applications. Phytochemical screening and antimicrobial activity of. Phytochemical screening was carried out and the in vitro. Phytochemical screening revealed the presence of phenols, sterols in petro leum ether and chloroform extracts, whereas tannins, flavonoids, coumarins, saponins and triterpenoids in methanol extract. All the plant extracts were quantitatively tested by using standard method for the presence of chemical constituents such as alkaloids, phenols, flavonoids, siponin, glycosides, steroids and terpenoids 1216 table 1. Collection and identification of plant materials the barks of healthy plant cinnamomum zeylanicum were collected from local areas of patwadangar near nainital, uttarakhand between april to may 2012 for phytochemical screening. Antioxidant activity was evaluated using dpph free radical scavenging assay. The methanolic stem extracts of cardiospermum halicacabum was evaluated for antibacterial.
The phytochemical tests were performed according to the method of brinda 10 and are presented in table 1. Petroleum ether extract exhibited the highest antifungal effica cy against all tested fungal species. Phytochemical screening affirmed the presence of alkaloids, flavonoids. Another 5 g of each plant material was extracted by infusion in 50 ml of distilled water. Tlc is a simple,quick and inexpensive procedure that indicates how. Phytochemical, antimicrobial potential and antifungal. Some of the bioactive substances that can be derived from plants are flavonoids, alkaloids, carotenoids, tannin, antioxidants and. Phytochemical screening refers to the extraction, screening and identification of the medicinally active substances found in plants. Collection and processing of plant material the plant material of aegle marmelos was collected in the month of june 2019 from the local area of bangalore, karnataka, india. Phytochemical screening the phytochemical analysis was carried out respectively on the three obtained extracts diethyl ether extract. The phytochemical screening of crude methanolic extracts of leaf and bark samples of g. Pdf phytochemical screening and antibacterial assay of the crude. Test for saponins one ml of the tepal extract was diluted with distilled water to 20 ml and shaken in a graduated cylinder for 15 minutes. The dendrogram was performed to detect the presence of the specific phytochemical group of diverse secondary metabolite classes in the methanolic plant extracts.
Preparation of plant extracts the leaves were air dried and crushed into small pieces using mortar and pestle and pulverized using an electric osterizer. The alcoholic and hydroalcoholic extracts of the root of prosopis cineraria were subjected to preliminary phytochemical screening to identify the constituents present in them 8, 9. Medicinal plants medicinal plants constitute an effective source of both traditional and modern medicines herbal medicine has been shown to have genuine utility about 80% of rural population depends on it as primary health care. The solvent was recovered using rotary evaporator and the extracts were dried in the oven at 600c for 3 minutes to remove any. Preliminary phytochemical analysis of the extracts of. Preliminary phytochemical screening of the crude extracts of wsroot revealed the presence of different kind of chemical groups summarized in table 1.
Preliminary phytochemical screening of different solvent. Natural crude drug extracts, isolated from plant species can be prolific sources for such new drugs 3. Extraction of secondary metabolites, phytochemical. Preliminary phytochemical screening and antioxidant. Dried extracts were stored at 4c for further analysis assam et al. Phytochemical screening of some plants used in herbal. Phyllanthus amarus, clerodendrum viscosum, ailanthus excelsa, syzigium cumini and cassia occidentalis by using polar solvent methanol, non polar hexane and aqueous extract.
Comparison of phytochemical screening and antioxidant activities. The selected plant part removed and then washed under running tap water to remove dirt. Medicinal plants are intrinsic components of traditional medicinal systems in treating. Antidiabetic activity and phytochemical screening of. Preliminary phytochemical analysis and extraction of crude drugs.
Qualitative phytochemical analysis preliminary phytochemical analysis was carried out for the extract as per standard methods described by brain and turner 1975 and evans 1996. Phytochemical analysis of medicinal plants occurring in. Phytochemical screening, in vitro antioxidant and antibacterial activities of methanolic extracts of capparis spionsa l. I hereby solemnly declare that the research work titled extraction of secondary metabolites, phytochemical screening and the analysis of antibacterial activity in stevia rebaudiana. Phytochemical screening of root, stem and leaves of cenchrus biflorus roxb sunita arora and ganesh kumar. Secondary metabolites, extraction, phytochemical screening, pharmacological activities. Tannins were detected in the leaf extract but not in the bark extract of g. The spectrum of coverage is broad encompassing methods and techniques relevant to. Preliminary phytochemical screening of the bark extracts of ficus nervosa showed the presence of chemical constituents like alkaloids, glycosides, sugars and carbohydrates. Phytochemical screening of some compounds from plant leaf. Extracts obtained were filtered, concentrated and allowed to dry till constant weight was obtained. Antimicrobial investigation of plant extracts and phytochemicals is. Qualitative phytochemical screening phytochemical analysis of each extract has been carriedout according to standard protocols anyasor et al. The extracts of these various plants shows the antimicrobial activity against various microorganisms and have effective for the treatments of alimentary canal.
The presence of bioactive compounds indicate the medicinal value of various extracts of wsplant, because their possible use as natural additives emerging from a growing tendency to replace. Phytochemical analysis of traditional medicinal plants and. The result for total phenol content was the highest in butanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract. Phytochemical screening of some compounds from plant leaf extracts of holoptelea integrifolia planch. The crude extracts thus obtained were used for further investigation of phytochemical screening, and antimicrobial evaluation.
The aim of the work is to ascertain the level of antimicrobial and antioxidant properties of different solvent extracts of senna occidentalis plant. Phytochemical screening, antimicrobial and antioxidant activities of the hexane, ethylacetate and methanol crude extracts of senna occidentalis l. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. Tlc is a technique with large applicability in the fields of plant material analysis. Introduction maesa lanceolata myrsinaceae is a wellknown plant and widely distributed in many parts of africa especially in. The phytochemical compounds detected are known to have medicinal importance. Extraction is the main step for the recovery and isolation of bioactive components. Phytochemical qualitative analysis the plant aqueous, ethanolic, acetone and methanolic extracts were screened for the presence of the phytochemical classes by using the standard following methods. Aly 1biology department, hafr albatin university, saudi arabia phytochemistry, medicinal and aromatic plants department, desert research center, elmataria, cairo, egypt. Detection of alkaloids extracts were dissolved individually in dilute hydrochloric acid and filtered. Phytochemical, antioxidant and antimicrobial properties of. Phytochemical investigations, extraction and thin layer.
Fresh plant samples were collected from three different zones i. The dried plant extracts were then redissolved in dimethyl sulfoxide and to get the solution of 10 mg10 ml for each extract which was subjected to analysis of in. Medicinal plants are the richest bioresource of drugs of traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk. Phytochemical analysis is devoted to the publication of original articles on the utilization of analytical methodology in the plant sciences. Stevia rebaudiana is a plant of the asteraceae family and native to paraguay. This plant according to folk medicine has been reported to be used for treatment of female sterility, dressing of sores and schistosomiasis. Pharmacognostical, physicochemical and phytochemical. Phytochemical studies have attracted the attention of plant scientists due to the development of new and sophisticated techniques. Pdf phytochemicals are secondary metabolites produced by all plants in which some has medicinal uses.
Preliminary phytochemical screening of different solvent extracts of some medicinal plants 1inas m. Ficus nervosa was collected, powdered and defatted with petroleum ether and then extracted successively with chloroform, ethyl acetate and 90% alcohol. Preliminary phytochemical screening, isolation and structural elucidation of chloroform leaf extracts of maesa lanceolata. Phytochemical screening and evaluation of pharmacological activity.
The extracts were subjected to preliminary phytochemical testing to detect the presence of different chemical groups. Twenty grams of each of the pulverized plant materials were mixed with 100ml of. Qualitative and quantitative phytochemical analysis of. Phytochemical screening and antimicrobial studies of.
Airdried and powdered plant materials were screened for the presence of saponins, tannins, alkaloids, flavonoids, triterpenoids, steroids, glycosides, saponins, gum, mucilage, carbohydrates. A preliminary phytochemical screening of different plant extracts aqueous. Phytochemical screening and hptlc fingerprint analysis of. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra. Phytochemical screening of selected medicinal plant.
The antimicrobial activity of the plant extract was figured out by disc. Extraction and phytochemical analysis of medicinal plants. Phytochemical screening, physicochemical properties, acute. Pdf phytochemical screening of some compounds from plant. Phytochemical screening of extract ensured the presence of carbohydrates, tannins, flavonoids, steroids and alkaloids. Antioxidant property of the spinach is due to the presence of bioactive compounds. Each plant sample was then air dried and milled to a coarse powder. The organic extraction was performed by soxhlet extraction method. For phytochemical analysis of plant extract thin layer chromatography and preliminary screening method of phytoconstitute by sofowara.
The antimicrobial activity of the plant extracts was determined using the well diffusion method against eleven selected pathogenic microbes and the minimum inhibitory concentrations mic were measured. Preliminary phytochemical screening of selected medicinal. Preliminary phytochemical screening was carried out using color forming and precipitating chemical reagents for detecting plant constituents from their extracts. Phytochemical screening and in vitro antimicrobial activity of various. Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids, saponins and steroids compounds. View phytochemical screening of medicinal plants research papers on academia. The two samples extracts were tested for the steroids, phenolic compounds, saponins, tannins, flavonoids and sugars. A 10 g of the powdered sample was extracted with ethanol by cold extraction.
Preparation of plant extracts these barks were washed with distilled water to remove the adhering dust particles. The screening of plant extracts and their products for antimicrobial activity has shown that higher plants represent a potential source of novel antibiotic prototypes. Pdf phytochemical analysis of leaf extract of phyllanthus fraternus. Manapparai, tiruchirappalli and thuraiyur, tiruchirappalli district, tamilnadu, india during 2012 20. Extraction of secondary metabolites, phytochemical screening and the. Gas chromatography and mass spectroscopy analysis and. The plant material was airdried for 15 days and stored at room temperature without exposure to direct sunlight. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay. The preliminary screening of the various extracts was carried out using standard. Phytochemical analysis and evaluation of leaf and root parts of the. Phytochemical screening the preliminary phytochemical analysis of the extracts carried out using aqueous and ethanolic extracts and on the powdered specimens using standard procedures to identify the various constituents described by sofowora 11, trease and. Pdf phytochemical screening of some compounds from plant leaf. Phytochemical investigation of leaves and fruits extracts. Phytochemical analysis of plant extracts free download as powerpoint presentation.
The results obtained from the tests were summarized in table table2, 2, indicating the presence of flavonoids, tannins, saponins, phenolic compounds and steroids. Preliminary phytochemical screening of the extracts revealed the presence of terpenoids, steroids, flavanoids, tannins, saponins and phenolic compounds. Phytochemical screening tests was conducted for five plant species and found that extract contains a variety of phytochemicals like saponins, tannins, flavonoids, terpenoids, glycosides and reducing sugars and among which there is higher level of precipitation for phenol and flavonoids. After shaking for 15 minutes, the extracts were filtered through whatmans filter paper and labelled as methanol extract. This process was allowed on repeatedly, for about 4 hours. The whole plant of petiveria alliacea was investigated for its pharmacognostic parameters viz, macroscopic, microscopic, physicochemical attributes, fluorescence analysis and phytochemical screening and the salient diagnostic features were also documented. Phytochemical analysis and antibacterial potential of.
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